Manufacture and extraction of nitrogenous products from products of animal origin



Patented May 17, 1932 UNITED" STATES PAT-ENT FFIQE munten kann, or rams, ramon maman am nxrahcrloo mnocnnous raonucfrs'raolr montrera or Amun. einem lolirawing. Applleation'nled July 5, 1924, Serial No. 724,540, and 'in France July 7, 19113;-flgeuevvcl-y y Y I uly28.' 192V8f 'y -A series of antiseptic substances that can be readily eliminated, carbon bisul hide in particular sindv in general allthe su stances hereinafter recited permit fish andl broadly i all products of animal origin containing, a

proteolytic ferment to be treated by autolysis r by hetero] is or by a combination of autolvsis and ofylsieterolysis.

" y the term heterolysis,

ogous to that'of autolysis; said action caused by proteolytic ferments introduce from without (heteroother) whereas autolysis is caused by ferments contained in the f. cells of the albuminoid substance itself. By the term auto-heterolysis is signified a blogical action caused by the presence and cooperation of both the ferments of the albuminoid itself and also the ferments introduced from without. in some instancessthe action of the ferments of the albuminoid undergoing treatment is not suiiiciently strong` to effeet the desired result, making it necessary to add extraneous ermen Such substances are:

1st H drocarbo'ns, benzene, as toluene-s. 2n hloruretted halogenous derivatives. i

(a) 'Fatty series:Y Chloroform--tetrachloi9 ride of carbon-dichlorethylene--trichlorethylene-tetrachloracetylenepentachlorethune-symmetrical and unsymmetrical di-y chlorethanes-chlrides of propyl, butyl and liquid homologues thereof-chlorides of a propylene, butyiene and liquid homologues (5th) -Aldehydessromiaihehyae and/its product of condensation with ammonia hexamethylenetetramines-aeetaldehyde chloral-acroleme-crotonic aldehyde furfuroL 'line-earthA as used her ein,. lo ssigniied a biotic or biological action, anall i(th) Ketones" of the fatty series or their mixture.

-v (7th) Phenolstphenol-cresolsand homologues-thereof pure or mixed, their halo enous and nitrated, alcoholated etc., derlvatives.

(9th) Quinolin audits homologues'. i The raw mixture of the bases from-tar distillation.

(8th) Biomides soluble,` alkaline and alkan II 'can the above mentioned 'bodies-'either singly or mixed with onea'nother or. againl with inert-bodies added. .i

. If I take carbon bisulphide 'as the-.easily eliminated antiseptic substance, I find that it renders proof against any microbian action a mass of matter that variesaccordin'g to the animal product treated in certain cases ive to tenfparts of carbon bisulphide per thousand o animal-matter are suicient 'forb eE- clnfc mal matter to be preserved'from any microhian action and to successfully undergo autosulphide-t r:- g care to stirV the mass thorlinee carbon bisulphidaone liter of this product permits 150 to 200 kilogramsof anior apparatus oughly, I then bring the mass tof be 'treated into,` a temperature suitable for auto-hetervats must be containedzin a chamber the walls van ledtoprovide devices for stirring th e during the advisable to provide so as-to avoid means of agglomerated cork or o'f ,1su1 },'1 V

temperatyrosine, tryptophane eta). o to make most valuable alimentary broths, to enter very usefully as a part into the food al course of operations. At the end of from 15 to Qll'days autoheterolysis is completed and I obtain a mass composed of various valuable elements which have to beI separatedl from one'another, wherefore it scaused to pass over very fine meshed and continuously moving sievcs.

In these sieves materials unalected by auto-heterolysis and more especially the's'keleton or bony rframework of the original material undergoing treatment.

Treated with tetrachloride of carbon, the

The liquid that has passed through the sieve and is of a rather thick consistency suggestive of glycerine has then to be filtered in a lilter press, or in vacuum etc.

I then obtain: y.

On the filter 'a complex fatty body which is highly valuable. for use in treating hides or which can be separated into oil and pulverulentnpowder.

The liquid which passes through the filter is of a fine brown or reddish colour. I can dry this product in any of theways now in industrial use and bring it to a asty condition or to a ver fine powder. his product has been freed. y air draught or blast from any traces of carbon bisulphide it may still contain. (Many other industrial moans suc as a current of steam etc. can be readily used to eliminate the antiseptic substance.)

The paste obtained is pleasantly palatable and rich in nitrogenous matters it contains in ap reciable quantity amino acids such as,

` It is calculated lowance of animals (especially for pig breed- M heterolysis, which layer can be separately collected by decanting.

ing)

In certain cases, a layer of oil is found on top of the matter obtained at the end of auto'- Quite obviously, centrifugal methods, or any other industrial means of separation, can be added to the above indicated ones.

In order to hasten auto-heterolysis and to treat animal matter, .I can' add, besides the proteolytic ferment contained in said matter, gastric or intestinal mucous membranes, pancreatine, papaine or any other additional proteolytic ferment'taken from the animal or from the vegetable kingdom and according to cases I effect autolysis, heterolysis or auto-heterolysis.

Having lnow particularly ascertained and will remain all the coarsedescribed the nature of my said invention as well as the manner in which the same is to be performed, I declare that what I claim is:

' 1. A process for the manufacture of nitrogenous products, comprising the steps of mixing carbon bisulphide with a protein product of animal origin and a proteolytic ferment other than such as maybe present in said product of animal origin; .subject- 'ing the m-ixturc to heat at a -moderate tem-A perature'for a' period long enough vto effect auto-digestion; and separating the liquid extract obtained thereby from the solid residue. 1 2. A process for the manufacture of nitrogenous products, comprising the steps of mixing carbon b-isulphide with a protein product of animal origin in the proportion of 5-10 parts of carbon -bisulphide per 1000 parts of the animal product.

3.- A process for the manufacture of nitrogenous products, comprising the steps of mixing carbon bisulphide with a protein product of animal origin and a proteolytic vferment other than such as may be present in said product of, animal origin; -subjecting the mixture to heat at a moderate temperature'so as to prevent destruction of said. ferment and for a period long enough to el'ect auto-di estion; and separating the liquid ortion-o the resultant product fromv the soli residue.

4. A process according to claim 3, in which the carbon bisulphide and the protein product of animal origin are mixed in the proportion of '5-10 parts of the `former per` 1000 h parts of the latter.v

5. The. process for trogenous products, comprising the steps of mixing an easily-remove le'gvolatile antisepthe manufacture of nitic substance with a protein product of animal origin gontaining a proteolytic ferment;

adding thereto' another proteolytic ferment; subjecting the final lmixture to heat at a moderate temperature so as to prevent destruction of said fel-ments and for a period long enou h to effect auto-.digestion and separating t e liquid portion of the resultant produ ct from the solid residue.

6. A process according to claim 5, in which the separated liquid portion is dried to powder form.

7. A process for the manufacture of nitrog- I enous products, comprising thesteps of mixin an easily removable volatile ant1septic su stance other than a compound of the har-` monic series to which nitro-chloroform belon with a protein product of animal origin an aproteolytic ferment other than such as may be present in said product of animal origin, su jecting the mixture toheat atamodcrate temperature for a period long enough to eiect digestion, and separating the liquid extract obtained thereby from the solid residue.

- 8. A process for the manufacture of nitrogenous products, comprising the steps of mixinfr an easily removable volatile antiseptic sulistance other than a compound of the harmonic series to which nitrochloroform belongs with a protein product of animal origin and i a proteolytic ferment other than such as may he present in said product of animal origin; subjecting the mixture to a moderate degree of heat such that said proteolytic ferment is not destroyed and for such a, period time as to eect auto-digestion of the organic substances, and separating the liquid extract obtained thereby from the solid residue. 9. A processforthcmanufacture ofnitrogl5 cnous products, comprising the steps of mixing an easily-removable volatile -antiseptic substance with a product of anim al origin and a proteolytic ferment other than such as may be present in said product of animal origin subjecting the mixture to heat at a moderate temperature so as to prevent destruction of said ferment and for a period long enough to etect auto-digestion; and separating the liquid portion of the resultant product from the solid residue.

10. A process for the manufacture of nitrogenous products, comprising the steps of mixing an easily-removable volatile antisep tic substance with a. product of animal origin Y l0 and a proteolytic ferment other than such as may be present in, said product of animal origin; subjectingthe mixture to heat. at a moderate temperature and for va period long enough to effect autowdigestion; and separating the liquid extract obtained thereby from the solid residue.

l1. A process for the manufacture of nitrogenous products, comprising the steps of mixing an easily-1'eu1o\'able volatile antiseptic substance. with a product of animal origin' and a proteolytic ferment other than such as may1 be.present in said product of animal origin; subjecting the mixture to heat at a moderate temperature and for a period long enough' to effect auto-digestion; separating the liquid extract obtained thereby from the solid residue; and finally drying the liquid extract to powder form.

12. A process accordingtoclaim 11, in which the product of animal origin is crushed previous to being mixed with the antiseptic substance.

In testimony whereof I aflx my signature.

MAURICE KAHN. 

